M. Thway1, M. J. Hsann1, L. M. May1, N. H. Win1 1University of Medicine 1,Pharmacology,Yangon, LANMADAW, Myanmar
Introduction: Hyperlipidemia is a group of disorders characterized by an excess of serum total cholesterol, excess LDL-C or excess triglycerides. Hyperlipidemia is a major modifiable risk factor for coronary heart disease and it is important to correct all causes of disease condition.Hibiscus sabdariffa Linn. known as Chin-baung-ni is a plant which belongs to the Malvaceae family and is widely cultivated in tropical and subtropical areas. Taking together these evidences of hypolipidemic efficacies of Hibiscus sabdariffa Linn., it was also worth to know lipid lowering and the sustained lipid lowering action of Hibiscus sabdariffa Linn. Moreover, it was so interesting to focus the effects of Hibiscus which grow in Myanmar climatic and ecological changes. Therefore this study was conducted to evaluate the effects of Hibiscus sabdariffa Linn. on serum lipid profile in hyperlipidemic subjects.
Methods: In this study, fourty-four hyperlipidemic human volunteers of both genders, who were between 20 – 60 years age group were chosen as subjects and they were randomly allocated into 3 g intake group and 10 g intake group. All subjects were requested to take one package of Hibiscus powder per day for 30 days. The package of Hibiscus powder was prepared in 250 mL of boiled water with sugar (5 g), steeped for 15 minute and it was drunk daily before lunch. Initial baseline levels were recorded and at 30 day of the study, 10 hour fasting blood samples were taken and analyzed for serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and then these biochemical parameters were analyzed again after two week washout period to know the sustainable lipid lowering effect. Enzymatic colorimetric test with Lipid Clearing Factor CHOD-PAP-method and Lipid Clearing Factor GPO-PAP method were used for determination of serum TC and TG levels respectively. HDL-C was determined by HUMAN Cholesterol liquicolour test Kit. Estimation of LDL cholesterol level was calculated by using method of Fridewald et al. (1972).
Results:
After 30 days consumption, it was found that no significant serum lipid level changes in 3 g daily intake group (P = > 0.05). However, 10 g daily intake group showed significant reduction in mean serum TC, TG, LDL-C levels by 16.13%, 19.33% and 22.33% respectively. Then, 12.3% elevation of mean serum HDL-C from baseline levels (P = 0.00) was seen. Concerned about sustainable effect in 10 g daily intake group, serum lipid profile levels returned nearly to the initial baseline levels after two week washout period. Therefore, 30 days consumption of Hibiscus is not sustainable after 2 week cessation of the intake.
Conclusion:Therefore, according to the obtained data, the higher dose of Hibiscus, daily 10 g intake gave significant lipid lowering effect, however the lower dose, daily 3 g intake have no significant lipid lowering effect